Human Sprouty 2 (SPRY2) activation kit by CRISPRa

CAT#: GA106981

SPRY2 CRISPRa kit - CRISPR gene activation of human sprouty RTK signaling antagonist 2

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: SPRY2
• Locus ID: 10253
• Kit Components:

  GA106981G1, SPRY2 gRNA vector 1 in pCas-Guide-CRISPRa

  GA106981G2, SPRY2 gRNA vector 2 in pCas-Guide-CRISPRa

  GA106981G3, SPRY2 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001318536, NM_001318537, NM_001318538, NM_005842
• Synonyms: hSPRY2; IGAN3
• Summary: This gene encodes a protein belonging to the sprouty family. The encoded protein contains a carboxyl-terminal cysteine-rich domain essential for the inhibitory activity on receptor tyrosine kinase signaling proteins and is required for growth factor stimulated translocation of the protein to membrane ruffles. In primary dermal endothelial cells this gene is transiently upregulated in response to fibroblast growth factor two. This protein is indirectly involved in the non-cell autonomous inhibitory effect on fibroblast growth factor two signaling. The protein interacts with Cas-Br-M (murine) ectropic retroviral transforming sequence, and can function as a bimodal regulator of epidermal growth factor receptor/mitogen-activated protein kinase signaling. This protein may play a role in alveoli branching during lung development as shown by a similar mouse protein. [provided by RefSeq, Jul 2008]