Human RGS14 activation kit by CRISPRa

CAT#: GA107287

RGS14 CRISPRa kit - CRISPR gene activation of human regulator of G protein signaling 14

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: RGS14
• Locus ID: 10636
• Kit Components:

  GA107287G1, RGS14 gRNA vector 1 in pCas-Guide-CRISPRa

  GA107287G2, RGS14 gRNA vector 2 in pCas-Guide-CRISPRa

  GA107287G3, RGS14 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_006480, NM_001366617, NM_001366618
• Synonyms: regulator of G-protein signaling 14; regulator of G-protein signalling 14
• Summary: This gene encodes a member of the regulator of G-protein signaling family. This protein contains one RGS domain, two Raf-like Ras-binding domains (RBDs), and one GoLoco domain. The protein attenuates the signaling activity of G-proteins by binding, through its GoLoco domain, to specific types of activated, GTP-bound G alpha subunits. Acting as a GTPase activating protein (GAP), the protein increases the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G-protein heterotrimers, thereby terminating the signal. Alternate transcriptional splice variants of this gene have been observed but have not been thoroughly characterized. [provided by RefSeq, Jul 2008]