Human Calpain 10 (CAPN10) activation kit by CRISPRa

CAT#: GA107657

CAPN10 CRISPRa kit - CRISPR gene activation of human calpain 10

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: CAPN10
• Locus ID: 11132
• Kit Components:

  GA107657G1, CAPN10 gRNA vector 1 in pCas-Guide-CRISPRa

  GA107657G2, CAPN10 gRNA vector 2 in pCas-Guide-CRISPRa

  GA107657G3, CAPN10 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_021251, NM_023083, NM_023084, NM_023085, NM_023086, NM_023087, NM_023088, NM_023089
• Synonyms: calcium-activated neutral protease; calpain 10; NIDDM1; OTTHUMP00000164509
• Summary: Calpains represent a ubiquitous, well-conserved family of calcium-dependent cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation; domain II, the protease domain; domain III, a linker domain of unknown function; and domain IV, the calmodulin-like calcium-binding domain. This gene encodes a large subunit. It is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. This gene is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM), and is located within the NIDDM1 region. Multiple alternative transcript variants have been described for this gene. [provided by RefSeq, Sep 2010]