Human PUF60 activation kit by CRISPRa

CAT#: GA107835

PUF60 CRISPRa kit - CRISPR gene activation of human poly(U) binding splicing factor 60

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: PUF60
• Locus ID: 22827
• Kit Components:

  GA107835G1, PUF60 gRNA vector 1 in pCas-Guide-CRISPRa

  GA107835G2, PUF60 gRNA vector 2 in pCas-Guide-CRISPRa

  GA107835G3, PUF60 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001136033, NM_001271096, NM_001271097, NM_001271098, NM_001271099, NM_001271100, NM_014281, NM_078480, NM_001362895, NM_001362897, NM_001362896
• Synonyms: FIR; RoBPI; SIAHBP1; VRJS
• Summary: This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation. The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter. This complex represses MYC transcription through the core-TFIIH basal transcription factor. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Aug 2012]