Human NEDD4 2 (NEDD4L) activation kit by CRISPRa

CAT#: GA108222

NEDD4L CRISPRa kit - CRISPR gene activation of human NEDD4 like E3 ubiquitin protein ligase

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: NEDD4L
• Locus ID: 23327
• Kit Components:

  GA108222G1, NEDD4L gRNA vector 1 in pCas-Guide-CRISPRa

  GA108222G2, NEDD4L gRNA vector 2 in pCas-Guide-CRISPRa

  GA108222G3, NEDD4L gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001144964, NM_001144965, NM_001144966, NM_001144967, NM_001144968, NM_001144969, NM_001144970, NM_001144971, NM_001243960, NM_015277
• Synonyms: hNEDD4-2; NEDD4-2; NEDD4.2; RSP5
• Summary: This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein mediates the ubiquitination of multiple target substrates and plays a critical role in epithelial sodium transport by regulating the cell surface expression of the epithelial sodium channel, ENaC. Single nucleotide polymorphisms in this gene may be associated with essential hypertension. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012]