Human SAP155 (SF3B1) activation kit by CRISPRa

CAT#: GA108316

SF3B1 CRISPRa kit - CRISPR gene activation of human splicing factor 3b subunit 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: SF3B1
• Locus ID: 23451
• Kit Components:

  GA108316G1, SF3B1 gRNA vector 1 in pCas-Guide-CRISPRa

  GA108316G2, SF3B1 gRNA vector 2 in pCas-Guide-CRISPRa

  GA108316G3, SF3B1 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001005526, NM_001308824, NM_012433
• Synonyms: Hsh155; MDS; PRP10; PRPF10; SAP155; SF3b155
• Summary: This gene encodes subunit 1 of the splicing factor 3b protein complex. Splicing factor 3b, together with splicing factor 3a and a 12S RNA unit, forms the U2 small nuclear ribonucleoproteins complex (U2 snRNP). The splicing factor 3b/3a complex binds pre-mRNA upstream of the intron's branch site in a sequence independent manner and may anchor the U2 snRNP to the pre-mRNA. Splicing factor 3b is also a component of the minor U12-type spliceosome. The carboxy-terminal two-thirds of subunit 1 have 22 non-identical, tandem HEAT repeats that form rod-like, helical structures. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]