Human PIK3R5 activation kit by CRISPRa

CAT#: GA108376

PIK3R5 CRISPRa kit - CRISPR gene activation of human phosphoinositide-3-kinase regulatory subunit 5

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: PIK3R5
• Locus ID: 23533
• Kit Components:

  GA108376G1, PIK3R5 gRNA vector 1 in pCas-Guide-CRISPRa

  GA108376G2, PIK3R5 gRNA vector 2 in pCas-Guide-CRISPRa

  GA108376G3, PIK3R5 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001142633, NM_001251851, NM_001251852, NM_001251853, NM_001251855, NM_014308
• Synonyms: F730038I15Rik; FOAP-2; p101; P101-PI3K
• Summary: Phosphatidylinositol 3-kinases (PI3Ks) phosphorylate the inositol ring of phosphatidylinositol at the 3-prime position, and play important roles in cell growth, proliferation, differentiation, motility, survival and intracellular trafficking. The PI3Ks are divided into three classes: I, II and III, and only the class I PI3Ks are involved in oncogenesis. This gene encodes the 101 kD regulatory subunit of the class I PI3K gamma complex, which is a dimeric enzyme, consisting of a 110 kD catalytic subunit gamma and a regulatory subunit of either 55, 87 or 101 kD. This protein recruits the catalytic subunit from the cytosol to the plasma membrane through high-affinity interaction with G-beta-gamma proteins. Multiple alternatively spliced transcript variants encoding two distinct isoforms have been found. [provided by RefSeq, Oct 2011]