Human NOB1 activation kit by CRISPRa

CAT#: GA109192

NOB1 CRISPRa kit - CRISPR gene activation of human NIN1 (RPN12) binding protein 1 homolog

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: NOB1
• Locus ID: 28987
• Kit Components:

  GA109192G1, NOB1 gRNA vector 1 in pCas-Guide-CRISPRa

  GA109192G2, NOB1 gRNA vector 2 in pCas-Guide-CRISPRa

  GA109192G3, NOB1 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_014062, NR_074074
• Synonyms: ART-4; MST158; MSTP158; NOB1P; PSMD8BP1
• Summary: In yeast, over 200 protein and RNA cofactors are required for ribosome assembly, and these are generally conserved in eukaryotes. These factors orchestrate modification and cleavage of the initial 35S precursor rRNA transcript into the mature 18S, 5.8S, and 25S rRNAs, folding of the rRNA, and binding of ribosomal proteins and 5S RNA. Nob1 is involved in pre-rRNA processing. In a late cytoplasmic processing step, Nob1 cleaves a 20S rRNA intermediate at cleavage site D to produce the mature 18S rRNA (Lamanna and Karbstein, 2009 [PubMed 19706509]). [supplied by OMIM, Nov 2010]