Human UHRF1 activation kit by CRISPRa

CAT#: GA109258

UHRF1 CRISPRa kit - CRISPR gene activation of human ubiquitin like with PHD and ring finger domains 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: UHRF1
• Locus ID: 29128
• Kit Components:

  GA109258G1, UHRF1 gRNA vector 1 in pCas-Guide-CRISPRa

  GA109258G2, UHRF1 gRNA vector 2 in pCas-Guide-CRISPRa

  GA109258G3, UHRF1 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001048201, NM_001290050, NM_001290051, NM_001290052, NM_013282
• Synonyms: hNP95; hUHRF1; huNp95; ICBP90; Np95; RNF106
• Summary: This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12. [provided by RefSeq, Feb 2014]