Human Phospholipase C epsilon 1 (PLCE1) activation kit by CRISPRa

CAT#: GA109642

PLCE1 CRISPRa kit - CRISPR gene activation of human phospholipase C epsilon 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: PLCE1
• Locus ID: 51196
• Kit Components:

  GA109642G1, PLCE1 gRNA vector 1 in pCas-Guide-CRISPRa

  GA109642G2, PLCE1 gRNA vector 2 in pCas-Guide-CRISPRa

  GA109642G3, PLCE1 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001165979, NM_001288989, NM_016341
• Synonyms: NPHS3; PLCE; PPLC
• Summary: This gene encodes a phospholipase enzyme that catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate to generate two second messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). These second messengers subsequently regulate various processes affecting cell growth, differentiation, and gene expression. This enzyme is regulated by small monomeric GTPases of the Ras and Rho families and by heterotrimeric G proteins. In addition to its phospholipase C catalytic activity, this enzyme has an N-terminal domain with guanine nucleotide exchange (GEF) activity. Mutations in this gene cause early-onset nephrotic syndrome; characterized by proteinuria, edema, and diffuse mesangial sclerosis or focal and segmental glomerulosclerosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2009]