Human YTHDF2 activation kit by CRISPRa

CAT#: GA109808

YTHDF2 CRISPRa kit - CRISPR gene activation of human YTH N6-methyladenosine RNA binding protein 2

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: YTHDF2
• Locus ID: 51441
• Kit Components:

  GA109808G1, YTHDF2 gRNA vector 1 in pCas-Guide-CRISPRa

  GA109808G2, YTHDF2 gRNA vector 2 in pCas-Guide-CRISPRa

  GA109808G3, YTHDF2 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001172828, NM_001173128, NM_016258
• Synonyms: CAHL; HGRG8; NY-REN-2
• Summary: This gene encodes a member of the YTH (YT521-B homology) superfamily containing YTH domain. The YTH domain is typical for the eukaryotes and is particularly abundant in plants. The YTH domain is usually located in the middle of the protein sequence and may function in binding to RNA. In addition to a YTH domain, this protein has a proline rich region which may be involved in signal transduction. An Alu-rich domain has been identified in one of the introns of this gene, which is thought to be associated with human longevity. In addition, reciprocal translocations between this gene and the Runx1 (AML1) gene on chromosome 21 has been observed in patients with acute myeloid leukemia. This gene was initially mapped to chromosome 14, which was later turned out to be a pseudogene. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. [provided by RefSeq, Oct 2012]