Human ALOXE3 activation kit by CRISPRa

CAT#: GA111830

ALOXE3 CRISPRa kit - CRISPR gene activation of human arachidonate lipoxygenase 3

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: ALOXE3
• Locus ID: 59344
• Kit Components:

  GA111830G1, ALOXE3 gRNA vector 1 in pCas-Guide-CRISPRa

  GA111830G2, ALOXE3 gRNA vector 2 in pCas-Guide-CRISPRa

  GA111830G3, ALOXE3 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001165960, NM_021628, NM_001369446
• Synonyms: ARCI3; E-LOX; eLOX-3; eLOX3
• Summary: This gene is a member of the lipoxygenase family, which are catabolized by arachidonic acid-derived compounds. The encoded enzyme is a hydroperoxide isomerase that synthesizes a unique type of epoxy alcohol (8R-hydroxy-11R,12R-epoxyeicosa-5Z,9E,14Z-trienoic acid) from 12R-hydroperoxyeicosatetraenoic acid (12R-HPETE). This epoxy alcohol can activate the the nuclear receptor peroxisome proliferator-activated receptor alpha (PPARalpha), which is implicated in epidermal differentiation. Loss of function of the enzyme encoded by this gene results in ichthyosis, implicating the function of this gene in the differentiation of human skin. This gene is part of a cluster of lipoxygenase genes on 17p13.1. Mutations in this gene result in nonbullous congenital ichthyosiform erythroderma (NCIE). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]