Human LIN7 (LIN7B) activation kit by CRISPRa

CAT#: GA111992

LIN7B CRISPRa kit - CRISPR gene activation of human lin-7 homolog B, crumbs cell polarity complex component

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: LIN7B
• Locus ID: 64130
• Kit Components:

  GA111992G1, LIN7B gRNA vector 1 in pCas-Guide-CRISPRa

  GA111992G2, LIN7B gRNA vector 2 in pCas-Guide-CRISPRa

  GA111992G3, LIN7B gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_022165, NM_001308419
• Synonyms: LIN-7B; MALS-2; MALS2; VELI2
• Summary: Plays a role in establishing and maintaining the asymmetric distribution of channels and receptors at the plasma membrane of polarized cells. Forms membrane-associated multiprotein complexes that may regulate delivery and recycling of proteins to the correct membrane domains. The tripartite complex composed of LIN7 (LIN7A, LIN7B or LIN7C), CASK and APBA1 may have the potential to couple synaptic vesicle exocytosis to cell adhesion in brain. Ensures the proper localization of GRIN2B (subunit 2B of the NMDA receptor) to neuronal postsynaptic density and may function in localizing synaptic vesicles at synapses where it is recruited by beta-catenin and cadherin. Required to localize Kir2 channels, GABA transporter (SLC6A12) and EGFR/ERBB1, ERBB2, ERBB3 and ERBB4 to the basolateral membrane of epithelial cells. May increase the amplitude of ASIC3 acid-evoked currents by stabilizing the channel at the cell surface. [UniProtKB/Swiss-Prot Function]