Human Endostatin (COL18A1) activation kit by CRISPRa

CAT#: GA112983

COL18A1 CRISPRa kit - CRISPR gene activation of human collagen type XVIII alpha 1 chain

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: COL18A1
• Locus ID: 80781
• Kit Components:

  GA112983G1, COL18A1 gRNA vector 1 in pCas-Guide-CRISPRa

  GA112983G2, COL18A1 gRNA vector 2 in pCas-Guide-CRISPRa

  GA112983G3, COL18A1 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_030582, NM_130444, NM_130445
• Synonyms: KNO; KNO1; KS
• Summary: This gene encodes the alpha chain of type XVIII collagen. This collagen is one of the multiplexins, extracellular matrix proteins that contain multiple triple-helix domains (collagenous domains) interrupted by non-collagenous domains. A long isoform of the protein has an N-terminal domain that is homologous to the extracellular part of frizzled receptors. Proteolytic processing at several endogenous cleavage sites in the C-terminal domain results in production of endostatin, a potent antiangiogenic protein that is able to inhibit angiogenesis and tumor growth. Mutations in this gene are associated with Knobloch syndrome. The main features of this syndrome involve retinal abnormalities, so type XVIII collagen may play an important role in retinal structure and in neural tube closure. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2014]