Human SETD7 activation kit by CRISPRa

CAT#: GA113003

SETD7 CRISPRa kit - CRISPR gene activation of human SET domain containing 7, histone lysine methyltransferase

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: SETD7
• Locus ID: 80854
• Kit Components:

  GA113003G1, SETD7 gRNA vector 1 in pCas-Guide-CRISPRa

  GA113003G2, SETD7 gRNA vector 2 in pCas-Guide-CRISPRa

  GA113003G3, SETD7 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_030648, NM_001306199, NM_001306200, NR_131339
• Synonyms: KMT7; SET7; SET7/9; SET9
• Summary: Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the [KR]-[STA]-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. [UniProtKB/Swiss-Prot Function]