Human EIF2A activation kit by CRISPRa

CAT#: GA113318

EIF2A CRISPRa kit - CRISPR gene activation of human eukaryotic translation initiation factor 2A

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: EIF2A
• Locus ID: 83939
• Kit Components:

  GA113318G1, EIF2A gRNA vector 1 in pCas-Guide-CRISPRa

  GA113318G2, EIF2A gRNA vector 2 in pCas-Guide-CRISPRa

  GA113318G3, EIF2A gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001319043, NM_001319044, NM_001319045, NM_001319046, NM_032025
• Synonyms: CDA02; EIF-2A; MST089; MSTP004; MSTP089
• Summary: This gene encodes a eukaryotic translation initiation factor that catalyzes the formation of puromycin-sensitive 80 S preinitiation complexes and the poly(U)-directed synthesis of polyphenylalanine at low concentrations of Mg2+. This gene should not be confused with eIF2-alpha (EIF2S1, Gene ID: 1965), the alpha subunit of the eIF2 translation initiation complex. Although both of these proteins function in binding initiator tRNA to the 40 S ribosomal subunit, the encoded protein does so in a codon-dependent manner, whereas eIF2 complex requires GTP. Alternative splicing of this gene results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jan 2016]