Human OBSCN activation kit by CRISPRa

CAT#: GA113338

OBSCN CRISPRa kit - CRISPR gene activation of human obscurin, cytoskeletal calmodulin and titin-interacting RhoGEF

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: OBSCN
• Locus ID: 84033
• Kit Components:

  GA113338G1, OBSCN gRNA vector 1 in pCas-Guide-CRISPRa

  GA113338G2, OBSCN gRNA vector 2 in pCas-Guide-CRISPRa

  GA113338G3, OBSCN gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001098623, NM_001271223, NM_052843
• Synonyms: ARHGEF30; UNC89
• Summary: The obscurin gene spans more than 150 kb, contains over 80 exons and encodes a protein of approximately 720 kDa. The encoded protein contains 68 Ig domains, 2 fibronectin domains, 1 calcium/calmodulin-binding domain, 1 RhoGEF domain with an associated PH domain, and 2 serine-threonine kinase domains. This protein belongs to the family of giant sacromeric signaling proteins that includes titin and nebulin, and may have a role in the organization of myofibrils during assembly and may mediate interactions between the sarcoplasmic reticulum and myofibrils. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]