Human PRPK (TP53RK) activation kit by CRISPRa

CAT#: GA114374

TP53RK CRISPRa kit - CRISPR gene activation of human TP53 regulating kinase

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: TP53RK
• Locus ID: 112858
• Kit Components:

  GA114374G1, TP53RK gRNA vector 1 in pCas-Guide-CRISPRa

  GA114374G2, TP53RK gRNA vector 2 in pCas-Guide-CRISPRa

  GA114374G3, TP53RK gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_033550
• Synonyms: BUD32; C20orf64; dJ101A2; Nori-2; Nori-2p; PRPK
• Summary: Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t(6)A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TC-AMP) to the N6 group of A37. TP53RK has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit OSGEP (By similarity). Atypical protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation. [UniProtKB/Swiss-Prot Function]