Human RASGRP 4 (RASGRP4) activation kit by CRISPRa

CAT#: GA114542

RASGRP4 CRISPRa kit - CRISPR gene activation of human RAS guanyl releasing protein 4

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: RASGRP4
• Locus ID: 115727
• Kit Components:

  GA114542G1, RASGRP4 gRNA vector 1 in pCas-Guide-CRISPRa

  GA114542G2, RASGRP4 gRNA vector 2 in pCas-Guide-CRISPRa

  GA114542G3, RASGRP4 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001146202, NM_001146203, NM_001146204, NM_001146205, NM_001146206, NM_001146207, NM_052949, NM_170602, NM_170603, NM_170604
• Synonyms: guanyl nucleotide releasing protein 4; RAS guanyl releasing protein 4
• Summary: The protein encoded by this gene is a member of the Ras guanyl nucleotide-releasing protein (RasGRP) family of Ras guanine nucleotide exchange factors. It contains a Ras exchange motif, a diacylglycerol-binding domain, and two calcium-binding EF hands. This protein was shown to activate H-Ras in a cation-dependent manner in vitro. Expression of this protein in myeloid cell lines was found to be correlated with elevated level of activated RAS protein, and the RAS activation can be greatly enhanced by phorbol ester treatment, which suggested a role of this protein in diacylglycerol regulated cell signaling pathways. Studies of a mast cell leukemia cell line expressing substantial amounts of abnormal transcripts of this gene indicated that this gene may play an important role in the final stages of mast cell development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2009]