Human STARD4 activation kit by CRISPRa

CAT#: GA115226

STARD4 CRISPRa kit - CRISPR gene activation of human StAR related lipid transfer domain containing 4

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: STARD4
• Locus ID: 134429
• Kit Components:

  GA115226G1, STARD4 gRNA vector 1 in pCas-Guide-CRISPRa

  GA115226G2, STARD4 gRNA vector 2 in pCas-Guide-CRISPRa

  GA115226G3, STARD4 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001308056, NM_001308057, NM_001308058, NM_001308059, NM_001308060, NM_001308061, NM_139164, NR_131752, NR_131753, NR_131754
• Summary: Cholesterol homeostasis is regulated, at least in part, by sterol regulatory element (SRE)-binding proteins (e.g., SREBP1; MIM 184756) and by liver X receptors (e.g., LXRA; MIM 602423). Upon sterol depletion, LXRs are inactive and SREBPs are cleaved, after which they bind promoter SREs and activate genes involved in cholesterol biosynthesis and uptake. Sterol transport is mediated by vesicles or by soluble protein carriers, such as steroidogenic acute regulatory protein (STAR; MIM 600617). STAR is homologous to a family of proteins containing a 200- to 210-amino acid STAR-related lipid transfer (START) domain, including STARD4 (Soccio et al., 2002 [PubMed 12011452]). [supplied by OMIM, Mar 2008]