Human BCDIN3D activation kit by CRISPRa

CAT#: GA115487

BCDIN3D CRISPRa kit - CRISPR gene activation of human BCDIN3 domain containing RNA methyltransferase

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: BCDIN3D
• Locus ID: 144233
• Kit Components:

  GA115487G1, BCDIN3D gRNA vector 1 in pCas-Guide-CRISPRa

  GA115487G2, BCDIN3D gRNA vector 2 in pCas-Guide-CRISPRa

  GA115487G3, BCDIN3D gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_181708
• Synonyms: BCDIN3 domain containing
• Summary: This gene encodes an RNA methyltransferase which belongs to the rossmann fold methyltransferase family, and serves as a 5'-methylphosphate capping enzyme that is specific for cytoplasmic histidyl tRNA. The encoded protein contains an S-adenosylmethionine binding domain and uses the methyl group donor, S-adenosylmethionine. This gene is overexpressed in breast cancer cells, and is related to the tumorigenic phenotype and poor prognosis of breast cancer. The encoded protein is thought to promote the cellular invasion of breast cancer cells, by downregulating the expression of tumor suppressor miRNAs through the dimethylation of the 5-monophosphate of the corresponding precursor miRNAs. [provided by RefSeq, Apr 2017]