Human GPBAR1 activation kit by CRISPRa

CAT#: GA115789

GPBAR1 CRISPRa kit - CRISPR gene activation of human G protein-coupled bile acid receptor 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: GPBAR1
• Locus ID: 151306
• Kit Components:

  GA115789G1, GPBAR1 gRNA vector 1 in pCas-Guide-CRISPRa

  GA115789G2, GPBAR1 gRNA vector 2 in pCas-Guide-CRISPRa

  GA115789G3, GPBAR1 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001077191, NM_001077194, NM_001321950, NM_170699
• Synonyms: BG37; GPCR19; GPR131; M-BAR; TGR5
• Summary: This gene encodes a member of the G protein-coupled receptor (GPCR) superfamily. This enzyme functions as a cell surface receptor for bile acids. Treatment of cells expressing this GPCR with bile acids induces the production of intracellular cAMP, activation of a MAP kinase signaling pathway, and internalization of the receptor. The receptor is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]