Human APOBEC3A activation kit by CRISPRa

CAT#: GA116344

APOBEC3A CRISPRa kit - CRISPR gene activation of human apolipoprotein B mRNA editing enzyme catalytic subunit 3A

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: APOBEC3A
• Locus ID: 200315
• Kit Components:

  GA116344G1, APOBEC3A gRNA vector 1 in pCas-Guide-CRISPRa

  GA116344G2, APOBEC3A gRNA vector 2 in pCas-Guide-CRISPRa

  GA116344G3, APOBEC3A gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001270406, NM_145699
• Synonyms: A3A; ARP3; bK150C2.1; PHRBN
• Summary: This gene is a member of the cytidine deaminase gene family. It is one of seven related genes or pseudogenes found in a cluster, thought to result from gene duplication, on chromosome 22. Members of the cluster encode proteins that are structurally and functionally related to the C to U RNA-editing cytidine deaminase APOBEC1. The protein encoded by this gene lacks the zinc binding activity of other family members. The protein plays a role in immunity, by restricting transmission of foreign DNA such as viruses. One mechanism of foreign DNA restriction is deamination of foreign double-stranded DNA cytidines to uridines, which leads to DNA degradation. However, other mechanisms are also thought to be involved, as anti-viral effect is not dependent on deaminase activity. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2012]