Human NEIL2 activation kit by CRISPRa

CAT#: GA116733

NEIL2 CRISPRa kit - CRISPR gene activation of human nei like DNA glycosylase 2

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: NEIL2
• Locus ID: 252969
• Kit Components:

  GA116733G1, NEIL2 gRNA vector 1 in pCas-Guide-CRISPRa

  GA116733G2, NEIL2 gRNA vector 2 in pCas-Guide-CRISPRa

  GA116733G3, NEIL2 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001135746, NM_001135747, NM_001135748, NM_145043, NM_001349439, NM_001349440, NM_001349441, NM_001349442, NR_146180, NR_146181, NR_146182
• Synonyms: NEH2; NEI2
• Summary: This gene encodes a member of the Fpg/Nei family of DNA glycosylases. These glycosylases initiate the first step in base excision repair by cleaving oxidatively damaged bases and introducing a DNA strand break via their abasic site lyase activity. This enzyme is primarily associated with DNA repair during transcription and acts prefentially on cytosine-derived lesions, particularly 5-hydroxyuracil and 5-hydroxycytosine. It contains an N-terminal catalytic domain, a hinge region, and a C-terminal DNA-binding domain with helix-two-turn-helix and zinc finger motifs. This enzyme interacts with the X-ray cross complementing factor 1 scaffold protein as part of a multi-protein DNA repair complex. A pseudogene of this gene has been identified. [provided by RefSeq, Mar 2017]