Human IBRDC2 (RNF144B) activation kit by CRISPRa

CAT#: GA116827

RNF144B CRISPRa kit - CRISPR gene activation of human ring finger protein 144B

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: RNF144B
• Locus ID: 255488
• Kit Components:

  GA116827G1, RNF144B gRNA vector 1 in pCas-Guide-CRISPRa

  GA116827G2, RNF144B gRNA vector 2 in pCas-Guide-CRISPRa

  GA116827G3, RNF144B gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_182757
• Synonyms: bA528A10.3; IBRDC2; p53RFP; PIR2
• Summary: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a p53/TP53-dependent but caspase-independent mechanism. However, its overexpression also produces a decrease of the ubiquitin-dependent stability of BAX, a pro-apoptotic protein, ultimately leading to protection of cell death; But, it is not an anti-apoptotic protein per se. [UniProtKB/Swiss-Prot Function]