Human NSE2 (NSMCE2) activation kit by CRISPRa

CAT#: GA117272

NSMCE2 CRISPRa kit - CRISPR gene activation of human NSE2 (MMS21) homolog, SMC5-SMC6 complex SUMO ligase

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: NSMCE2
• Locus ID: 286053
• Kit Components:

  GA117272G1, NSMCE2 gRNA vector 1 in pCas-Guide-CRISPRa

  GA117272G2, NSMCE2 gRNA vector 2 in pCas-Guide-CRISPRa

  GA117272G3, NSMCE2 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_173685, NM_001349485, NM_001349486, NM_001349487, NR_146191, NR_146192
• Synonyms: C8orf36; MMS21; NSE2; ZMIZ7
• Summary: This gene encodes a member of a family of E3 small ubiquitin-related modifier (SUMO) ligases that mediates the attachment of a SUMO protein to proteins involved in nuclear transport, transcription, chromosome segregation and DNA repair. The encoded protein is part of the structural maintenance of chromosomes (SMC) 5/6 complex which plays a key role genome maintenance, facilitating chromosome segregation and suppressing mitotic recombination. A knockout of the orthologous mouse gene is lethal prior to embryonic day 10.5. Naturally occurring mutations in this gene, that abolish the SUMO ligase activity, are associated with primordial dwarfism and extreme insulin resistance. [provided by RefSeq, Mar 2017]