Mouse Chrne activation kit by CRISPRa

CAT#: GA200038

Chrne CRISPRa kit - CRISPR gene activation of mouse cholinergic receptor, nicotinic, epsilon polypeptide

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Chrne
• Locus ID: 11448
• Kit Components:

  GA200038G1, Chrne gRNA vector 1 in pCas-Guide-CRISPRa

  GA200038G2, Chrne gRNA vector 2 in pCas-Guide-CRISPRa

  GA200038G3, Chrne gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_009603
• Synonyms: AChrepsilon; Acre; nAChRE
• Summary: This gene encodes the epsilon subunit of the muscle-derived nicotinic acetylcholine receptor, a pentameric neurotransmitter receptor and member of the ligand-gated ion channel superfamily. The acetylcholine receptor changes subunit composition shortly after birth when the epsilon subunit replaces the gamma subunit seen in embryonic receptors. In mice, deficiency of this gene can lead to a decline in the number of nicotinic acetylcholine receptors at neuromuscular junctions and causes progressive muscle weakness, atrophy and premature death. Mutations in this gene serve as a pathophysiological model for human congenital myasthenia. Several alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Nov 2012]