Mouse Asah1 activation kit by CRISPRa

CAT#: GA200295

Asah1 CRISPRa kit - CRISPR gene activation of mouse N-acylsphingosine amidohydrolase 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Asah1
• Locus ID: 11886
• Kit Components:

  GA200295G1, Asah1 gRNA vector 1 in pCas-Guide-CRISPRa

  GA200295G2, Asah1 gRNA vector 2 in pCas-Guide-CRISPRa

  GA200295G3, Asah1 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_019734
• Synonyms: 2310081N20Rik; AC; Asah
• Summary: This gene encodes acid ceramidase, an enzyme that plays a central role in ceramide metabolism. The encoded protein undergoes proteolytic processing to generate a heterodimeric enzyme comprised of alpha and beta subunits that catalyzes the hydrolysis of sphingolipid ceramide into sphingosine and free fatty acid. The homozygous disruption of this gene leads to embryonic lethality in mice whereas the heterozygous animals exhibit a progressive lipid storage disease phenotype. [provided by RefSeq, Oct 2015]