Mouse Col3a1 activation kit by CRISPRa

CAT#: GA200813

Col3a1 CRISPRa kit - CRISPR gene activation of mouse collagen, type III, alpha 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Col3a1
• Locus ID: 12825
• Kit Components:

  GA200813G1, Col3a1 gRNA vector 1 in pCas-Guide-CRISPRa

  GA200813G2, Col3a1 gRNA vector 2 in pCas-Guide-CRISPRa

  GA200813G3, Col3a1 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_009930
• Synonyms: AW550625; Col3a-1; Tsk-2; Tsk2
• Summary: This gene encodes the alpha-1 subunit of the fibril-forming type III collagen found in bone, cartilage, dentin, tendon, bone marrow stroma and other connective tissue. The encoded protein forms homotrimeric type III procollagen that undergoes proteolytic processing during fibril formation. A majority of mice lacking the encoded protein die within two days of birth but about 5% of the animals survive to adulthood. The surviving mice exhibit severe cortical malformation and experience significantly shorter lifespan. The mutant mouse named "tight skin 2" exhibiting systemic sclerosis phenotype was found to harbor a missense point mutation in this gene. A pseudogene of this gene has been defined on chromosome 8. [provided by RefSeq, Nov 2015]