Mouse Dbt activation kit by CRISPRa

CAT#: GA201025

Dbt CRISPRa kit - CRISPR gene activation of mouse dihydrolipoamide branched chain transacylase E2

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Dbt
• Locus ID: 13171
• Kit Components:

  GA201025G1, Dbt gRNA vector 1 in pCas-Guide-CRISPRa

  GA201025G2, Dbt gRNA vector 2 in pCas-Guide-CRISPRa

  GA201025G3, Dbt gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_010022, NM_001357344
• Synonyms: D3Wsu60e
• Summary: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component. [UniProtKB/Swiss-Prot Function]