Mouse Eif4a1 activation kit by CRISPRa

CAT#: GA201214

Eif4a1 CRISPRa kit - CRISPR gene activation of mouse eukaryotic translation initiation factor 4A1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Eif4a1
• Locus ID: 13681
• Kit Components:

  GA201214G1, Eif4a1 gRNA vector 1 in pCas-Guide-CRISPRa

  GA201214G2, Eif4a1 gRNA vector 2 in pCas-Guide-CRISPRa

  GA201214G3, Eif4a1 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001159375, NM_144958
• Synonyms: BM-010; Ddx2a; Eif4
• Summary: ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon. [UniProtKB/Swiss-Prot Function]