Mouse Eif4e activation kit by CRISPRa

CAT#: GA201216

Eif4e CRISPRa kit - CRISPR gene activation of mouse eukaryotic translation initiation factor 4E

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Eif4e
• Locus ID: 13684
• Kit Components:

  GA201216G1, Eif4e gRNA vector 1 in pCas-Guide-CRISPRa

  GA201216G2, Eif4e gRNA vector 2 in pCas-Guide-CRISPRa

  GA201216G3, Eif4e gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001313980, NM_007917
• Synonyms: EG668879; eIF-4E; Eif4e-ps; If4e
• Summary: This gene encodes a component of the eukaryotic translation initiation factor 4F complex, which recognizes the 7-methylguanosine cap structure at the 5' end of messenger RNAs. The encoded protein aids in translation initiation by recruiting ribosomes to the 5'-cap structure. Association of this protein with the 4F complex is the rate-limiting step in translation initiation. This gene acts as a proto-oncogene, and its expression and activation is associated with transformation and tumorigenesis. It has also been associated with autism spectrum disorders. Consistently, knockout of this gene results in increased translation of neuroligins, postsynaptic proteins linked to autism spectrum disorders. Pseudogenes of this gene are found on other chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]