Mouse Fzd5 activation kit by CRISPRa

CAT#: GA201496

Fzd5 CRISPRa kit - CRISPR gene activation of mouse frizzled class receptor 5

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Fzd5
• Locus ID: 14367
• Kit Components:

  GA201496G1, Fzd5 gRNA vector 1 in pCas-Guide-CRISPRa

  GA201496G2, Fzd5 gRNA vector 2 in pCas-Guide-CRISPRa

  GA201496G3, Fzd5 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001042659, NM_022721
• Synonyms: 5330434N09Rik; AI427138; Fz-5; Fz5; mFz5
• Summary: Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Plays a role in yolk sac angiogenesis and in placental vascularization. Binds to Wnt2, Wnt10B, Wnt5A, but not to Wnt2B or Wnt4. [UniProtKB/Swiss-Prot Function]