Mouse Galt activation kit by CRISPRa

CAT#: GA201541

Galt CRISPRa kit - CRISPR gene activation of mouse galactose-1-phosphate uridyl transferase

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Galt
• Locus ID: 14430
• Kit Components:

  GA201541G1, Galt gRNA vector 1 in pCas-Guide-CRISPRa

  GA201541G2, Galt gRNA vector 2 in pCas-Guide-CRISPRa

  GA201541G3, Galt gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001302511, NM_016658, NM_001369135, NM_001369140, NM_001369141, NM_001369142, NM_001369143, NM_001369144, NM_001369147
• Synonyms: AW553376
• Summary: The protein encoded by this gene is the second enzyme in the Leloir pathway, the metabolic pathway for D-galactose catabolism. It catalyzes the conversion of galactose-1-phosphate and uridine diphosphate-glucose to glucose-1-phosphate and uridine diphosphate galactose. Deficiency of this enzyme causes the genetic metabolic disorder galactosemia. Mice lacking this protein accumulate high levels of galactose and galactose-1 phosphate but are viable and fertile. This protein is negatively regulated through signaling by the polypeptide hormone prolactin, specifically via the short isoform of the prolactin receptor and the transcription factor Forkhead box O3. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]