Mouse Htr2c activation kit by CRISPRa

CAT#: GA202031

Htr2c CRISPRa kit - CRISPR gene activation of mouse 5-hydroxytryptamine (serotonin) receptor 2C

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Htr2c
• Locus ID: 15560
• Kit Components:

  GA202031G1, Htr2c gRNA vector 1 in pCas-Guide-CRISPRa

  GA202031G2, Htr2c gRNA vector 2 in pCas-Guide-CRISPRa

  GA202031G3, Htr2c gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_008312
• Synonyms: 5-HT2C; 5-HT2cR; 5-HTR2C; 5HT1c; Htr1c; SR1
• Summary: Serotonin (5-hydroxytryptamine, 5-HT), a neurotransmitter, elicits a wide array of physiological effects by binding to several receptor subtypes, including the 5-HT2 family of seven-transmembrane-spanning, G-protein-coupled receptors, which activate phospholipase C and D signaling pathways. This gene encodes the 2C subtype of serotonin receptor and its mRNA is subject to multiple RNA editing events, where genomically encoded adenosine residues are converted to inosines. RNA editing is predicted to alter amino acids within the second intracellular loop of the 5-HT2C receptor and generate receptor isoforms that differ in their ability to interact with G proteins and the activation of phospholipase C and D signaling cascades, thus modulating serotonergic neurotransmission in the central nervous system. Studies in rodents show altered patterns of RNA editing in response to drug treatments and stressful situations. [provided by RefSeq, Jul 2008]