Mouse Mcm7 activation kit by CRISPRa

CAT#: GA202608

Mcm7 CRISPRa kit - CRISPR gene activation of mouse minichromosome maintenance complex component 7

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Mcm7
• Locus ID: 17220
• Kit Components:

  GA202608G1, Mcm7 gRNA vector 1 in pCas-Guide-CRISPRa

  GA202608G2, Mcm7 gRNA vector 2 in pCas-Guide-CRISPRa

  GA202608G3, Mcm7 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_008568, NM_001356576, NM_001356577
• Synonyms: AI747533; mCDC47; Mcmd7
• Summary: Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage. [UniProtKB/Swiss-Prot Function]