Mouse Npc1 activation kit by CRISPRa

CAT#: GA202945

Npc1 CRISPRa kit - CRISPR gene activation of mouse NPC intracellular cholesterol transporter 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Npc1
• Locus ID: 18145
• Kit Components:

  GA202945G1, Npc1 gRNA vector 1 in pCas-Guide-CRISPRa

  GA202945G2, Npc1 gRNA vector 2 in pCas-Guide-CRISPRa

  GA202945G3, Npc1 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_008720
• Synonyms: A430089E03Rik; C85354; D18Ertd139e; D18Ertd723e; lcsd; nmf164; spm
• Summary: Intracellular cholesterol transporter which acts in concert with NPC2 and plays an important role in the egress of cholesterol from the endosomal/lysosomal compartment. Both NPC1 and NPC2 function as the cellular 'tag team duo' (TTD) to catalyze the mobilization of cholesterol within the multivesicular environment of the late endosome (LE) to effect egress through the limiting bilayer of the LE. NPC2 binds unesterified cholesterol that has been released from LDLs in the lumen of the late endosomes/lysosomes and transfers it to the cholesterol-binding pocket of the N-terminal domain of NPC1. Cholesterol binds to NPC1 with the hydroxyl group buried in the binding pocket and is exported from the limiting membrane of late endosomes/ lysosomes to the ER and plasma membrane by an unknown mechanism. Binds oxysterol with higher affinity than cholesterol. May play a role in vesicular trafficking in glia, a process that may be crucial for maintaining the structural and functional integrity of nerve terminals. [UniProtKB/Swiss-Prot Function]