Mouse Oaz1 activation kit by CRISPRa

CAT#: GA203000

Oaz1 CRISPRa kit - CRISPR gene activation of mouse ornithine decarboxylase antizyme 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Oaz1
• Locus ID: 18245
• Kit Components:

  GA203000G1, Oaz1 gRNA vector 1 in pCas-Guide-CRISPRa

  GA203000G2, Oaz1 gRNA vector 2 in pCas-Guide-CRISPRa

  GA203000G3, Oaz1 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001301034, NM_008753
• Synonyms: Antizyme; AZ-1; AZ1; Oaz; ODC-Az
• Summary: The protein encoded by this gene belongs to the ornithine decarboxylase antizyme family, which plays a role in cell growth and proliferation by regulating intracellular polyamine levels. Expression of antizymes requires +1 ribosomal frameshifting, which is enhanced by high levels of polyamines. Antizymes in turn bind to and inhibit ornithine decarboxylase (ODC), the key enzyme in polyamine biosynthesis; thus, completing the auto-regulatory circuit. This gene encodes antizyme 1, the first member of the antizyme family, that has broad tissue distribution, and negatively regulates intracellular polyamine levels by binding to and targeting ODC for degradation, as well as inhibiting polyamine uptake. Antizyme 1 mRNA contains two potential in-frame AUGs; and studies in rat suggest that alternative use of the two translation initiation sites results in N-terminally distinct protein isoforms with different subcellular localization. Alternatively spliced transcript variants have also been noted for this gene. [provided by RefSeq, Dec 2014]