Mouse Enpp1 activation kit by CRISPRa

CAT#: GA203163

Enpp1 CRISPRa kit - CRISPR gene activation of mouse ectonucleotide pyrophosphatase/phosphodiesterase 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Enpp1
• Locus ID: 18605
• Kit Components:

  GA203163G1, Enpp1 gRNA vector 1 in pCas-Guide-CRISPRa

  GA203163G2, Enpp1 gRNA vector 2 in pCas-Guide-CRISPRa

  GA203163G3, Enpp1 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001308327, NM_001308329, NM_008813
• Synonyms: 4833416E15Rik; AI428932; C76301; CD203c; E-NPP 1; E-NPP1; Ly-41; M6S1; NPP1; Npps; PC-1; Pca
• Summary: This gene encodes a member of the nucleoside pyrophosphatase/phosphodiesterase family of enzymes that catalyzes the hydrolysis of pyrophosphate and phosphodiester bonds in nucleotide triphosphates and oligonucleotides, respectively, to generate nucleoside 5'-monophosphates. The encoded protein is a type II transmembrane glycoprotein that negatively regulates bone mineralization. Mice harboring a nonsense mutation in this gene, termed tiptoe walking (ttw), exhibit ectopic ossification of the spinal ligaments. The encoded protein binds to the insulin receptor, inhibits downstream signaling events and induces insulin resistance and glucose tolerance. This gene is located adjacent to a paralog on chromosome 10. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]