Mouse Pla2g4a activation kit by CRISPRa

CAT#: GA203267

Pla2g4a CRISPRa kit - CRISPR gene activation of mouse phospholipase A2, group IVA (cytosolic, calcium-dependent)

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Pla2g4a
• Locus ID: 18783
• Kit Components:

  GA203267G1, Pla2g4a gRNA vector 1 in pCas-Guide-CRISPRa

  GA203267G2, Pla2g4a gRNA vector 2 in pCas-Guide-CRISPRa

  GA203267G3, Pla2g4a gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001305632, NM_008869
• Synonyms: cPLA2; cPLA2-alpha; cPLA2alpha; Pla2g4
• Summary: The protein encoded by this gene is a member of the phospholipase A2 group IV family. This enzyme hydrolyzes membrane phospholipids, thereby releasing the polyunsaturated fatty acid, arachidonic acid. Arachidonic acid is further metabolized into eicosanoids such as leukotrienes, thromboxanes and prostaglandins, that play important roles in regulating diverse biological processes such as inflammatory responses, membrane and actin dynamics, and tumorigenesis. A rise in intracellular calcium levels results in binding of calcium to the C2 domain of this protein, and triggers the translocation from the cytosol to intracellular membranes, including the Golgi apparatus. Disruption of this gene in mice led to decreased levels of eicosonaoids and platelet-activating factor, decreased allergic symptoms, and impaired reproductive ability in females. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Mar 2015]