Mouse Pparg activation kit by CRISPRa

CAT#: GA203341

Pparg CRISPRa kit - CRISPR gene activation of mouse peroxisome proliferator activated receptor gamma

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Pparg
• Locus ID: 19016
• Kit Components:

  GA203341G1, Pparg gRNA vector 1 in pCas-Guide-CRISPRa

  GA203341G2, Pparg gRNA vector 2 in pCas-Guide-CRISPRa

  GA203341G3, Pparg gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001127330, NM_001308352, NM_001308354, NM_011146
• Synonyms: Nr1c3; PPAR-gamma; PPAR-gamma2; PPARgamma; PPARgamma2
• Summary: This gene encodes a nuclear receptor protein belonging to the peroxisome proliferator-activated receptor (Ppar) family. The encoded protein is a ligand-activated transcription factor that is involved in the regulation of adipocyte differentiation and glucose homeostasis. The encoded protein forms a heterodimer with retinoid X receptors and binds to DNA motifs termed "peroxisome proliferator response elements" to either activate or inhibit gene expression. Mice lacking the encoded protein die at an embryonic stage due to severe defects in placental vascularization. When the embryos lacking this gene are supplemented with healthy placentas, the mutants survive to term, but succumb to lipodystrophy and multiple hemorrhages. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Apr 2015]