Mouse Srebf1 activation kit by CRISPRa

CAT#: GA204103

Srebf1 CRISPRa kit - CRISPR gene activation of mouse sterol regulatory element binding transcription factor 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Srebf1
• Locus ID: 20787
• Kit Components:

  GA204103G1, Srebf1 gRNA vector 1 in pCas-Guide-CRISPRa

  GA204103G2, Srebf1 gRNA vector 2 in pCas-Guide-CRISPRa

  GA204103G3, Srebf1 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001313979, NM_011480, NM_001358314, NM_001358315
• Synonyms: ADD1; bHLHd1; SREBP-1a; SREBP1; SREBP1c
• Summary: This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. Alternatively spliced transcript variants have been characterized for this gene. [provided by RefSeq, Nov 2017]