Mouse Twist1 activation kit by CRISPRa

CAT#: GA204498

Twist1 CRISPRa kit - CRISPR gene activation of mouse twist basic helix-loop-helix transcription factor 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Twist1
• Locus ID: 22160
• Kit Components:

  GA204498G1, Twist1 gRNA vector 1 in pCas-Guide-CRISPRa

  GA204498G2, Twist1 gRNA vector 2 in pCas-Guide-CRISPRa

  GA204498G3, Twist1 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_011658
• Synonyms: bHLHa38; M-Twist; Pde; pdt; Ska10; Ska; Twist
• Summary: Basic helix-loop-helix (bHLH) transcription factors have been implicated in cell lineage determination and differentiation. This gene encodes a bHLH transcription factor that is evolutionarily conserved from invertebrates to humans, and was originally identified in Drosophila as an essential gene involved in early mesoderm development and dorsal-ventral patterning in the embryo. This protein plays a role in cancer by regulating the epithelial-mesenchymal transition (EMT), a process that is critical for metastasis initiation, and promoting tumor progression. Mutations in the human gene are associated with Saethre-Chotzen syndrome (SCS). Mice with heterozygous mutations in this gene exhibit cranofacial and structural defects similar to those seen in human SCS patients. [provided by RefSeq, Sep 2015]