Mouse Nt5e activation kit by CRISPRa

CAT#: GA204833

Nt5e CRISPRa kit - CRISPR gene activation of mouse 5' nucleotidase, ecto

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Nt5e
• Locus ID: 23959
• Kit Components:

  GA204833G1, Nt5e gRNA vector 1 in pCas-Guide-CRISPRa

  GA204833G2, Nt5e gRNA vector 2 in pCas-Guide-CRISPRa

  GA204833G3, Nt5e gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_011851
• Synonyms: 5'-NT; 2210401F01Rik; AI447961; CD73; eNT; NT; Nt5
• Summary: This gene encodes a membrane-bound nucleotidase that hydrolyzes extracellular nucleoside monophosphates. The encoded preproprotein undergoes proteolytic processing to generate to a functional, homodimeric enzyme that preferentially uses adenosine monophosphate as a substrate to generate free adenosine. Mice lacking the encoded protein exhibit a significantly reduced fall in stop flow pressure and superficial nephron glomerular filtration rate in response to a saturating increase of tubular perfusion flow. [provided by RefSeq, Sep 2016]