Mouse Oas1b activation kit by CRISPRa

CAT#: GA204835

Oas1b CRISPRa kit - CRISPR gene activation of mouse 2'-5' oligoadenylate synthetase 1B

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Oas1b
• Locus ID: 23961
• Kit Components:

  GA204835G1, Oas1b gRNA vector 1 in pCas-Guide-CRISPRa

  GA204835G2, Oas1b gRNA vector 2 in pCas-Guide-CRISPRa

  GA204835G3, Oas1b gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001083925, NM_011853, NR_003507
• Synonyms: Flv; L1; Mmu-L1; Oas1; Oias-2; Oias2; Wnv
• Summary: This gene is a member of the 2'-5' oligoA synthetase family, which are clustered on chromosome 5. The encoded protein functions in the interferon-regulated OAS/RNase L system, which mediates RNA decay as part of the innate antiviral immunity pathway. The protein binds double-stranded RNA and oligomerizes ATP, which activate the single-stranded RNA cleavage enzyme RNase L. This protein mediates resistance to flaviviruses such as West Nile virus. The majority of wild mouse strains produce a functional protein and are resistant to flavivirus infection, whereas some inbred mouse strains including the strain of the reference genome, C57BL/6J, contain a premature stop codon that inactivates this gene product. [provided by RefSeq, Jul 2008]