Mouse Nlrp5 activation kit by CRISPRa

CAT#: GA204842

Nlrp5 CRISPRa kit - CRISPR gene activation of mouse NLR family, pyrin domain containing 5

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Nlrp5
• Locus ID: 23968
• Kit Components:

  GA204842G1, Nlrp5 gRNA vector 1 in pCas-Guide-CRISPRa

  GA204842G2, Nlrp5 gRNA vector 2 in pCas-Guide-CRISPRa

  GA204842G3, Nlrp5 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001039143, NM_001305857, NM_011860, NR_131241, NR_131242
• Synonyms: Mater; Nalp5; Op1; PAN11
• Summary: This gene encodes a member of the NACHT, leucine-rich repeat, and pyrin domain containing family. Members of this family have a pyrin domain at the N-terminus, a central NACHT domain, and a C-terminal leucine-rich repeat domain. This gene encodes a maternal-effect factor that is essential for early embryonic development in the mouse. Homozygous null mutant females are sterile, and embryos die following the first cleavage. This gene is required for endoplasmic reticulum redistribution and calcium homeostasis in oocytes. In addition, ovulated oocytes mutant for this gene have abnormal mitochondrial localization and increased mitochondrial activity, which results in mitochondrial damage and early embryonic lethality. Pseudogenes of this gene have been found on chromosomes 7 and 12. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]