Mouse Pla2g10 activation kit by CRISPRa

CAT#: GA205006

Pla2g10 CRISPRa kit - CRISPR gene activation of mouse phospholipase A2, group X

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Pla2g10
• Locus ID: 26565
• Kit Components:

  GA205006G1, Pla2g10 gRNA vector 1 in pCas-Guide-CRISPRa

  GA205006G2, Pla2g10 gRNA vector 2 in pCas-Guide-CRISPRa

  GA205006G3, Pla2g10 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001291009, NM_011987, NR_110990
• Synonyms: GX sPLA2; PLA2GX; sPLA2-X
• Summary: This gene encodes a member of the phospholipase A2 family of lipolytic enzymes that hydrolyzes glycerophospholipids to produce free fatty acids and lysophospholipids. The encoded protein undergoes proteolytic processing to generate a calcium-dependent enzyme that plays pivotal roles in the liberation of arachidonic acid from membrane phospholipids leading to the production of various inflammatory lipid mediators, such as prostaglandins. In response to myocardial ischemia/reperfusion, mice lacking the encoded protein display a reduction in myocardial infarct size partly through the suppression of neutorphil cytotoxic activities. Alternative splicing results in multiple transcript variants encoding different isoforms. All of these isoforms may undergo similar processing to generate the mature protein. [provided by RefSeq, Jul 2015]