Mouse Suz12 activation kit by CRISPRa

CAT#: GA205495

Suz12 CRISPRa kit - CRISPR gene activation of mouse SUZ12 polycomb repressive complex 2 subunit

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Suz12
• Locus ID: 52615
• Kit Components:

  GA205495G1, Suz12 gRNA vector 1 in pCas-Guide-CRISPRa

  GA205495G2, Suz12 gRNA vector 2 in pCas-Guide-CRISPRa

  GA205495G3, Suz12 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001163018, NM_199196
• Synonyms: 2610028O16Rik; AI195385; AU016842; AW536442; D11Ertd530e; mKIAA0160
• Summary: This gene encodes a core component of the polycomb repressive complex 2 (PRC2) that also includes, at least, embryonic ectoderm development protein (EED) and enhancer of zeste homolog 1 or 2 (EZH1 or EZH2). Through the methyltransferase activity of EZH1 or EZH2, the PRC2 complex methylates Lys9 and Lys27 of histone 3 and Lys26 of histone 1, leading to recruitment of the PRC1 complex, histone 2A ubiquitylation and transcriptional repression of the target genes. This gene product is essential for the activity and integrity of the PRC2 complex, and is required for X chromosome inactivation, stem cell maintenance and differentiation. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2009]