Mouse Atp5d activation kit by CRISPRa

CAT#: GA206541

Atp5d CRISPRa kit - CRISPR gene activation of mouse ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Atp5d
• Locus ID: 66043
• Kit Components:

  GA206541G1, Atp5d gRNA vector 1 in pCas-Guide-CRISPRa

  GA206541G2, Atp5d gRNA vector 2 in pCas-Guide-CRISPRa

  GA206541G3, Atp5d gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001347092, NM_025313
• Synonyms: 0610008F14Rik; 1500000I11Rik; AA960090; AI876556; AU020773; C85518
• Summary: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP turnover in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Part of the complex F(1) domain and of the central stalk which is part of the complex rotary element. Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. [UniProtKB/Swiss-Prot Function]