Mouse Scnm1 activation kit by CRISPRa

CAT#: GA208110

Scnm1 CRISPRa kit - CRISPR gene activation of mouse sodium channel modifier 1

Product Images

Specifications

Product Data

• Format: 3gRNAs, 1 scramble ctrl and 1 enhancer vector
• Symbol: Scnm1
• Locus ID: 69269
• Kit Components:

  GA208110G1, Scnm1 gRNA vector 1 in pCas-Guide-CRISPRa

  GA208110G2, Scnm1 gRNA vector 2 in pCas-Guide-CRISPRa

  GA208110G3, Scnm1 gRNA vector 3 in pCas-Guide-CRISPRa

  1 CRISPRa-Enhancer vector, SKU GE100056

  1 CRISPRa scramble vector, SKU GE100058

• Disclaimer: The kit is designed based on the best knowledge of CRISPa SAM technology. The efficiency of the activation can be affected by many factors, including nucleosome occupancy status, chromatin structure and the gene expression level of the target, etc.

Reference Data

• RefSeq: NM_001163573, NM_027013
• Synonyms: 3110001I17Rik; Scnm1-ps
• Summary: Mutations in the voltage-gated sodium channel gene Scn8a lead to neurological problems in mice. For one particular mutation, Scn8amedJ, mice live to adulthood but have tremors and muscle weakness, among other problems, in all strains except those derived from C57BL6 mice. In these strains, the product of the Scnm1 gene (229 aa) partially overcomes the effects of the Scn8amedJ mutation. However, in C57BL6-derived mice, a one nt change in the penultimate exon creates a premature stop codon, truncating the Scnm1 protein at 186 aa. This truncated protein lacks the ability to overcome the effects of the Scn8amedJ mutation, and these mice suffer paralysis and juvenile death. [provided by RefSeq, Jul 2009]